Enabling Multidimensional Liquid Chromatography for Analysis and Purification of Pharmaceuticals via Computer-assisted Method Development Software

Thursday, March 11, 2021: 2:10 PM - 2:30 PM

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Description

Two-Dimensional Liquid Chromatography (2D-LC) now makes separation and analysis of very complex mixtures achievable by improving peak capacity and selectivity dramatically. Despite being such a powerful chromatographic tool, 2D-LC technology can often be seen as a “promising toy,” that in its current state, unleashes a series of arduous method development activities poorly suited for a fast-paced industrial environment. However, many chromatographers believe that the lack of a systematic way to effectively optimize 2D-LC separations is a missing link in securing the viability of 2D-LC as a mainstay technique for industrial applications. Although computer-assisted 1D-LC modeling has reached a mature stage across both industrial and academic sectors, 2D-LC platforms have not been elevated to this same standard. This presentation will describe a new computer-assisted modeling approach that dramatically simplifies both offline and online 2D-LC method development. Our methodology centers on mapping the separation landscape of pharmaceutically relevant mixtures across both dimensions using LC Simulator (ACD/Labs) software as an initial analytical framework to analysis and purification workflows, enabling simple and straightforward 2D-LC method development for both analytical and preparative applications. This computer-assisted modeling approach streamlines facile 2D-LC method development and optimization towards more efficient and industrious offline/online multidimensional chromatography workflows in the development of new drug substances.

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Additional Info

Keywords: Please select up to 4 keywords ONLY:
Chromatography - Other,Method Development,Prep Chromatography/Isolation/Purification